Irangi rya Mindray 5 - Igice cyo gusesengura Hematology kirimo amarangi yihariye ya polymethine na acide nucleic aside fluorescent irangi ihuza cyane na ADN selile na RNA. Izi reagent zerekana uturemangingo twamaraso yera, reticulocytes, na platine imbere muri selile itemba, bigafasha gutandukanya ibintu byinshi bitandukanye binyuze mumashanyarazi ya laser yamashanyarazi no gutahura fluorescence.

Ikirangantego cya Hematologiya ni iki?

Imiti ya Hematology irangi / irangi kubisesengura byikora ni ibisubizo byimiti irimo fluorofores organic, surfactants, na osmotic stabilisateur. Bitandukanye na mikorosikopi yintoki yamaraso, iyi reagent irangi yamazi ikorera imbere ifunze - sisitemu yimikorere ya cytometrie.

Iyo batewe inshinge mucyumba cyo gutahura, irangi ryinjira mubice bigize selile byateguwe na lyse reagents kandi bigahuza stoichiometricique na acide nucleic acide. Iyo ihuye na lazeri ya semiconductor imbere muri selile itemba, fluorophores ihambiriye itanga urumuri rwa fluorescent urumuri rujyanye na aside nucleic selile.

Uruhare mu Gutondekanya Akagari / Kumenya

Irangi rya Fluorescent rituma itandukanyirizo ryiza rya selile yamaraso igoye:
Nucleic Acide Label:Fluorophores ihuza imirongo ya RNA na ADN. Ingirabuzimafatizo zifite ibikorwa byinshi byo guhindagurika cyangwa ibyiciro bitarakura bikurura irangi ryinshi, bitanga ibimenyetso bikomeye bya fluorescent.
Igisekuru cya Scattergram:Guhuza uruhande rutatanya urumuri hamwe na fluorescence ubukana butera - ibipimo bitandukanya.
Kugenzura Akagari kadasanzwe:Imyirondoro itandukanye ya fluorescent itandukanya lymphocytes idasanzwe, granulocytes idakuze, hamwe na selile zitukura ziva mumasemburo yera akuze.

Imiyoboro Yerekana

Umuyoboro	Izina ry'umuyoboro / Intego	Imikorere ya Biologiya & Uburyo bwo gupima
Umuyoboro wa WNR	Akagari Cyera Nucleated & RBC Umuyoboro	Gupima umubare WBC kubara no kubara NRBC. Basophile itandukanijwe ikwirakwizwa kumubiri mugihe acide NRBC nucleic acide yanditseho fluorescent.
Umuyoboro wa WDF	Umuyoboro Wera Utandukanye	Itandukanya Neutrophile, Lymphocytes, Monocytes, na Eosinofile. Gupima Granulocytes idakuze binyuze muri acide nucleic aside fluorescent irangi rihuza ubukana.
Umuyoboro wa WPC	Umuyoboro Wera Utugari	Mugaragaza ya progenitor selile, lymphocytes zidasanzwe, hamwe no guturika. Koresha uburyo bwihariye bwa membrane permeability reaction ihujwe no gufata irangi kugirango itandukanye selile ziturika.
SHAKA Umuyoboro	Umuyoboro wa Reticulocyte	Ibipimo Igipimo cya Reticulocyte, kubara byimazeyo, Igice cya Reticulocyte kidakuze, hamwe na selile yamaraso itukura ihwanye na RNA fluorescent irangi.
Umuyoboro wa PLT	Umuyoboro mwiza wa platel	Itanga plaque optique ibara ukoresheje irangi rya fluorescent irangi rya platine RNA, ikabuza kwivanga kwa microcytike RBCs cyangwa imyanda.

Isesengura ryisesengura

Automatic 5-Igice cya Laser Hematology Urukurikirane:Byakozwe kuri sisitemu ya fluidic ikoresha semiconductor laser fluorescence itemba cytometrie.

Hejuru - Iyinjiza Modular Hematology Workstations:Bihujwe na capat yikora - gutobora icyitegererezo hamwe no guhora wikoreza imirongo ya fluidic.

Kwinjiza Hematologiya - Sisitemu yo gukora sisitemu:Byemejwe kumurongo utaziguye reagent sisitemu idafite ibimenyetso bitwara cyangwa itandukaniro ryumuvuduko.

Ibicuruzwa byihariye

Parameter	Tekiniki ya tekiniki / Amakuru
Ubwoko bw'imikorere	Amazi yibanze ya fluorescent irangi
Base Fluorophore	Polymethine / Nucleic aside fluorescent irangi
Umuhengeri	633 nm - 635 nm (Lazeri itukura ya semiconductor)
pH Urwego (dogere 25)	7.00 – 8.50
Imyitwarire (dogere 25)	1.20 - 2,50 mS / cm
Ubucucike (dogere 20)	1.005 - 1.020 g / cm³
Fungura - Vial Stabilite	Iminsi 60 (dogere 2 - dogere 30 mububiko bwijimye)
Ubuzima bwa Shelf budafunguwe	Amezi 18
Ubushyuhe Ububiko	Impamyabumenyi 2 - dogere 30 (Kurinda urumuri no gukonja)
Kurungurura	Kurungurura binyuze muri 0.2 µm membrane

Porogaramu & Reagent Imikorere

Isesengura ritandukanye rya WNR:Yinjijwe mu cyumba cya reaction ya WNR kugirango yandike nuclea selile itukura na nuclei yamaraso yera nyuma ya cytoplazme.

WDF 5-Igice cya WBC Gutandukana:Imikoranire na WBC subpopulations kumuyoboro wa WDF, itanga cluster itandukanye ya lymphocytes, monocytes, neutrophile, na eosinofile.

Precursor & Guturika Akagari:Yateguwe kugirango ihitemo neza ihinduranya lipid membrane mu muyoboro wa WPC, ituma hamenyekana umubare w’utugari tutarakura.

Reticulocyte RNA Umubare:Ihuza na cytoplasmic ribosomal RNA muri reticulocytes, itanga kubara ibyiciro byo gukura kwa reticulocyte.

Kubara Amashanyarazi meza:Ihindura ibice bya RNA by'imbere mu maraso kugira ngo bibyare neza mu gihe kitari gito -.

Ububiko / Ububiko

Ingingo	Ibisobanuro
Gupakira bisanzwe	12 mL x 1 icupa / agasanduku, 42 mL x 1 icupa / agasanduku
Icupa	Umucyo - ikingira amber fluor icupa rya HDPE
Ubushyuhe Ububiko	Impamyabumenyi 2 kugeza kuri 30
Ibisabwa byo gutwara abantu	Irinde gukonja no kuyobora izuba

Bifitanye isano na Hematology Reagents

Umuti ushimishije kubice 5 bigize abasesengura Hematology:Isotonic iyobora buffer igisubizo cyamaraso sample dilution hamwe no guhagarika urusaku rwinyuma.

Lyse Reagent kubice 5 bigize abasesengura Hematology:Surfactant - ishingiye kuri lysing igisubizo cyo gutoranya erythrocyte membrane.

Igisubizo cyibanze cyogusukura:Isuku y'amazi yo gutunganya umurongo wa fluidic no gukuraho ububiko bwa protein.

Isuku:Alkaline hypochlorite probe yoza reagent ya sample probe deproteinisation.

Ibibazo

Ikibazo: Nigute reagent ya fluorescent irangi itandukaniye he na reagent ya maraso gakondo?

Igisubizo: Gusiga irangi gakondo bisaba gukama intoki kumurongo wibirahure kugirango bisuzumwe neza kuri microscope. Isesengura ryikora ryikora reagent ni irangi ryamazi ya fluorescent yagenewe ibyumba byerekana reaction. Bahuza na selile RNA / ADN muguhagarika amazi, bagatanga ibimenyetso byurumuri byagaragaye na laser flow cytometry optique.

Ikibazo: Ese urumuri rugira ingaruka kumikorere yibikorwa bya buri munsi?

Igisubizo: Yego. Fluorofore ikora itesha agaciro igihe kirekire kumurika cyangwa urumuri UV rudasanzwe. Reagents zapakiwe mumucyo - ikingira amber kandi igomba kuguma imbere mugice gifunze reagent mugihe ikora.

Ikibazo: Ni ubuhe bushyuhe bwo kubika busabwa mugihe cyo gutwara no kubika ububiko?

Igisubizo: Ububiko bwa reagent hagati ya dogere 2 na dogere 30. Ububiko buri munsi ya dogere 0 butera imvura ya fluorophore. Ubushyuhe buri hejuru ya dogere 35 bwihutisha ibikorwa byangirika.

Ikibazo: Nigute ubufindo - kugeza - uburinganire buhoraho ingaruka zitatanye?

Igisubizo: Guhindagurika muburyo bwo gusiga irangi bitera gukwirakwiza cluster ihindagurika, biganisha kumarembera. Buri gice cyo kubyaza umusaruro kigenzurwa na fluorophore, igeragezwa rya spekrofoforometrike, hamwe na matrix ihuza kugirango ikomeze itatanye.

Ikibazo: Ni ubuhe buryo bwo gupakira bukoreshwa muguhuza amazi?

Igisubizo: Irangi ryirangi ritangwa mumacupa ya fluor ya HDPE ifunze hamwe na cap adaptate yagenewe imirongo ifata amazi, birinda guhumeka no kwanduzwa.

Ibirangantego: Irangi rya mindray Abasesenguzi ba hematologiya ibice 5, Ubushinwa irangi rya mindray Ibice 5 byabasesenguzi ba hematologiya, abatanga isoko